Murine DCs were used to assess viability and uptake. B3Z, BMDCs, and DCs were used to assess cross-presentation and DC maturation (measured as IL-12 secretion and CD80 and CD86 expression).C57BL/6 mice bearing PancO2 tumours were used to assess anticancer effects of therapeutic vaccine injections given subcutaneously 7 days and in the intra-inguinal lymph nodes 14 days after tumour inoculation.OT-1 mice were used to isolate splenocytes. Here, CD80 is linked to neoplasm.