Since the ECS may control the release of mediators involved in headache [21,24], as a further test in this study we examined the effect of peripherally administered FAAH inhibitor URB597 and tooth pulp stimulation on mRNA levels of selected mediators, including endocannabinoids and their receptors, in the brain structures involved in the processing of pain signals from the orofacial area in rats. This evidence concerns the gene FAAH and Headache.