The results of KEGG pathway analysis for the downregulated DEPs between group S and group H are shown in Fig. 8B. The top three pathways were mineral absorption (major proteins: ATOX1 and CYBRD1), African trypanosomiasis (major proteins: ICAM1 and FAS), shown in Fig. 10, and central carbon metabolism in cancer (major proteins: GLS and G6PD). The gene discussed is ATOX1; the disease is cancer.