Furthermore, a ChIP-qPCR assay showed that GW treatment increased PPARδ binding to 3 of 4 predicted PPARδ binding sites in the murine Ccl2 promoter in murine KC PDAC cells stably transduced with a DDK-tagged PPARδ-expressing lentivirus (Supplementary Fig. 9f, Fig. 6m) and murine KC PDAC cells (Supplementary Fig. 9g), and also increased PPARδ binding to the predicted PPARδ binding site in the human CCL2 promoter of Panc-1 PDAC cells (Supplementary Fig. 9h, i). This evidence concerns the gene CCL2 and keratoconus.