To gain mechanistic insights into how SIRT6 mediated the effects of ER mimetics during cerebral ischemia, shNC or shSIRT6 lentivirus-infected HAPI cells and BMVECs were pre-treated with vehicle control, metformin (10 μM) or resveratrol (10 nM) for 12 h, followed by OGD/R treatment. This evidence concerns the gene SIRT6 and brain ischemia.