Importantly, OT‐I CD8 T cells that escape negative selection to the low avidity TRAs RIP‐mQ4R7 and RIP‐mT4 potently induce autoimmune diabetes upon immunization with OVAp or infection with OVA‐expressing Listeria monocytogenes (Koehli et al., 2014). This evidence concerns the gene CD8A and infection.