HTSJD treats high-fat diet-induced obesity and oligospermia by activating the PI3K-AKT signaling pathway and inhibiting the JNK MAPK signaling pathway, which alleviates obesity and blood lipids, increasing sperm motility and semen concentration, preventing inflammatory infiltration within the testes. This evidence concerns the gene MAPK8 and obesity due to melanocortin 4 receptor deficiency.