Figures 3C–E shows bar and bubble plots of the top 25 enriched GO functions and KEGG pathways, respectively. The GO functions related to obesity and oligoasthenozoospermia include reactive oxygen species metabolic process, germ cell development, hormone activity, oxidoreductase activity, synaptonemal structure, and male germ cell nucleus, and others. The top 25 pathways included the HIF-1 signaling pathway, FoxO pathway, PI3K-AKT signaling pathway, MAPK pathway, and Steroid hormone biosynthesis (Figure 3F). The gene discussed is AKT1; the disease is obesity due to melanocortin 4 receptor deficiency.