Anti-POSTN autoantibody can diagnose ESCC patients from normal controls in most subgroups except for patients with family tumor history and moderate and high differentiation, and it showed marginal difference in diagnosing ESCC patients younger than 60 years old (p = 0.0491) (Figures 5A–N). The gene discussed is POSTN; the disease is esophageal squamous cell carcinoma.