In cultured 3T3-L1 adipocytes, prolonged ET-1 treatment has also been shown to cause insulin resistance, in an ETAR-mediated process (95, 96), which is consistent with another study where prolonged ET-1 stimulation enhanced lipolysis in differentiated subcutaneous white adipocytes, again mediated by ETAR (97). This evidence concerns the gene EDN1 and Insulin resistance.