We co-transfected the reporter construct with either Atoh1 or Gfi1 constructs in DAOY cells, a cerebellar medulloblastoma cell line, and performed luciferase reporter assays with either an Atoh1 construct alone or co-expression of Atoh1 and Gfi1. The reporter activity was significantly higher when Atoh1 and Gfi1 vectors were co-transfected compared to Atoh1 alone (Fig. 6a). The gene discussed is GFI1; the disease is medulloblastoma.