Meanwhile, it is worth noting that the phosphorylation level of EphA3 was significantly up-regulated by the stimulation of exogenous ephrin-A5 ligand in both stable DU145 cell lines (Fig. 5F), suggesting that ephrin-A5 can not only promote the phosphorylation of EphA3 but also make EphA3 play an anti-tumor effect in vitro. This evidence concerns the gene EPHA3 and neoplasm.