Moreover, 4T1/Ubr5−/− shCdc73 cells, when injected intravenously into mice, completely regained the WT capacity to metastasize to the lungs, as measured by the number of metastatic nodules in the lungs (Fig. 4H), tumor cell colonies grown in culture dishes in the presence of 6-thioguanine (Fig. 4I), and metastatic foci visualized by hematoxylin and eosin (H&E) staining (Fig. 4J). Here, UBR5 is linked to neoplasm.