To study the location of SAS4 during the Plasmodium life cycle, we generated a C-terminal tagged SAS4-GFP transgenic line using rodent malaria model Plasmodium berghei, by inserting an in-frame gfp coding sequence at the 3′ end of the endogenous sas4 locus using single homologous recombination (Fig S1B). This evidence concerns the gene CPAP and malaria.