To determine if RIPK1 mediates MLKL phosphorylation in ZBP1-deficient astrocytes following infection, we treated ZBP1+/+ and ZBP1−/− derived astrocytes with the RIPK1 inhibitor, GSK963, during infection with the HSV-1(MacIntyre), HSV-1(F)-ICP6-RHIM Mut, and HSV-1(F) strains. The gene discussed is ZBP1; the disease is infection.