While the decreased cell death induced by HSV-1(F)-ICP6-RHIM Mut in ZBP1−/− astrocytes correlates with a significant reduction in the level of phosphorylated MLKL (Fig. 3B), decreased cell death seen following infection with HSV-1(F) occurred despite similar levels of phosphorylated MLKL to those seen in ZBP1+/+ cells, suggesting that necroptosis is not solely responsible for HSV-1-induced cell death. The gene discussed is ZBP1; the disease is infection.