CD274 and neoplasm: Thus; tumor samples, before and after neoadjuvant chemotherapy, of 60 patients with locally advanced gastric cancer were retrospectively identified and analyzed by multiplex immunohistochemistry, with a panel including PD-1 and PD-L1 [19]: following neoadjuvant chemotherapy, the overall median expression levels of PD1 and PD-L1 were significantly increased; moreover, high upregulation levels of these checkpoint molecules were correlated with survival benefits.