In accordance with the data obtained from MCF7 cells, increased phosphorylation of H2AX (Supplementary Figures S3A,B), blockage of DNA replication indicated by reduced EdU incorporation was also evident in U2OS and Saos-2 osteosarcoma cell lines treated with 11 μM and higher concentrations of compound AF615 (Supplementary Figures S3C,D). The gene discussed is H2AX; the disease is osteosarcoma.