Finally, in agreement with the above elaborated bioinformatic analyses, molecular and cellular evidence showed higher mRNA expression of TGF-β1 in two human HCC cell lines (Huh7 and HepG2) than in the normal human hepatic cell line (LO2) (Figure 5A) and in HCC tissues than in the corresponding paired non-tumorous liver tissues (Figure 5B), which was confirmed by immunofluorescent staining at protein level, with TGF-β1 expressed in cytoplasm (Figure 5C). Here, TGFB1 is linked to hepatocellular carcinoma.