MBP and ischemic stroke: The control PBS group exhibited a remarkable loss of MBP+ fibers and increase of SMI32+ demyelinated axons (resulting in a significant increase of SMI32/MBP immunofluorescent intensity ratio) in the corpus callosum, striatum and cortex after 28 days of tMCAO compared to the sham group (p < 0.01, Fig. 3A), suggesting ischemic stroke could induce profound white matter injury.