To further verify if PTRF exacerbated neuronal insults after ischemic stroke in vivo (Figure 7B), we use of the corner turn test to quantify neurological severity in the mice after cerebral I/R injury, which showed that the lower percentage of left turn was calculated in AAV-hSyn-sh-PTRF-transfected mice (Figure 7C). This evidence concerns the gene RIC8B and ischemic stroke.