Transmission electron microscopy (TEM) revealed a high presence of autophagosomes in the cerebral ischemic penumbra of the mice following cerebral I/R injury, whereas the stroke-triggered accumulation of autophagosomes was no significant difference in the cerebral ischemic penumbra between the AAV-hSyn-sh-Scra and AAV-hSyn-sh-PTRF pre-injected mice co-treated with AACOCF3 after cerebral I/R injury, consistent with the in vitro observations (Figure 6A-B and Figure S7A). This evidence concerns the gene RIC8B and Stroke.