To investigate the potential therapeutic benefit of Olig2-OPCs, we used a transient middle cerebral artery occlusion (tMCAO) rat model of ischemic stroke, Olig2-OPCs, and control-OPCs were frozen, thawed, and allowed to recover for 24-48 h before transplantation. This evidence concerns the gene OLIG2 and cerebral artery occlusion.