At the different developmental stages associated with infection of PWN, the expression levels of KLF normalized by RPL7 and TER individually, or RPL7 + TER were different with COX7 and RPS5. When normalized by RPS5, the expression of KLF increased in every developmental stage (L5, P1, P5, P10, AF and AM), and significantly decreased in AF and AM stages normalized by COX7. The gene discussed is RPS5; the disease is atrial fibrillation.