Moreover, studies show that the increase in calpain-2 levels during obesity leads to degradation of Atg-7, resulting in defective autophagy; also, knockdown of Atg-5 results in an increase in the intracellular triglyceride content, thus alleviating steatotic condition.66,67 In the present study, RA was found to restore the expression of the abovementioned genes, indicating its efficacy in regulating autophagy, the effect which was similar to that observed in a study performed with resveratrol.70 Here, CAPN2 is linked to obesity due to melanocortin 4 receptor deficiency.