A total of six cases of mixed infection of different assemblages were identified, including B (at the tpi locus) + E (at the SSU rRNA locus) in one cow, A (at the tpi locus) + E (at the SSU rRNA and bg loci) in one sheep, A (at the tpi locus) + E (at the SSU rRNA locus) in one sika deer, and C (at the SSU rRNA locus) + D (at the gdh locus) in one dog, as well as A + E in one cow and one sheep by assemblage-specific nested PCR assays targeting the tpi gene (Table 3). The gene discussed is TPI1; the disease is infection.