To assess the functional impact of IL-1 signalling on Treg cells, we performed bulk-RNA sequencing of blood Treg cells and IL1R1− and IL1R1+ HNSCC Treg cells after culture with anti-CD3/CD28/CD2 beads with or without IL-1 for 2 days. The gene discussed is IL1R1; the disease is head and neck squamous cell carcinoma.