It has been shown that too liberal infusion in septic shock causes degradation of glycocalyx and results in deterioration of its barrier function.21-24 In opposite, fluid restriction aiming at prevention of hypervolemia can protect EG by attenuating the release of atrial natriuretic peptide, which induces glycocalyx digestion mediated by matrix metalloproteinase.29 The gene discussed is NPPA; the disease is septic shock.