The microinjection of rmnd5a-mo into one blastomere at the two-cell stage resulted in developmental alterations, including brain anomalies reminiscent of occipital encephalocele and severe eye anomalies reminiscent of microphthalmia (Fig. 6E,F; Table S5) (Vogel et al., 2012). The gene discussed is RMND5A; the disease is occipital encephalocele.