In addition, we also found that the application of Gem could overactivate NF-κB, in line with other studies 10, 11, but treatment with alizarin not only significantly inhibited the activity of NF-κB in pancreatic cancer cells but also obviously downregulated the increase in NF-κB caused by Gem when used in combination. This evidence concerns the gene NFKB1 and pancreatic neoplasm.