Compared with control mice injected with normal saline (vehicle), mice treated with DOX exhibited impairment of cardiac function with dramatic decreases in EF and FS (Figure S1B), a reduction in the heart weight to tibia length (HW/TL) ratio (Figure S1C), increases in the serum levels of heart injury and heart failure markers (cTNT, LDH, CK-MB, and NT-proBNP) (Figure S1D), and massive cardiomyocyte apoptosis (Figure S1E and F). The gene discussed is NPPB; the disease is heart failure.