MPO and colitis: As been reported by Krawisz et al., the determination of MPO activity in the intestine is a simple biochemical assay that can be used to quantitate inflammation, thus MPO is a useful marker of acute inflammation in colonic tissues from animals with experimental colitis.27 The increased level of MPO in colon homogenate reflects the neutrophilic leukocytosis, the presence of inflammation, and the degrees of neutrophil infiltration.