It is of interest to explore the biological and clinical significance of genetic variants, this study evaluated the genetic polymorphisms of HSF1, HSF2 and UBE2I genes in human and tried to find out their involvement in the pathogenesis of male infertility.This study depicted the presence of rs78202224 variant (rare) genotype of HSF1 gene exclusively in 23 DNA samples out of 958 subjects. The gene discussed is UBE2I; the disease is male infertility.