AKT1 and neoplasm: With the synergistic effect of AKT and c-Met, rapid liver tumor development with hepatocytic differentiation has been observed as our previous study described.24 To investigate whether celastrol has oncotherapeutic effects in an earlier phase of tumor development, the mice were intraperitoneally treated daily with either vehicle control or celastrol (1.0 and 2.0 mg kg−1), 4 weeks after the hydrodynamic transfection had been finished.