To first determine whether GCN5L1 depletion‐induced glutaminolysis predisposes to HCC development, we subjected GCN5L1flox/flox (control) and GCN5L1flox/flox‐albumin‐Cre (GCN5L1 LKO) mice to a single dose of DEN at 2 weeks of postnatal life. Here, BLOC1S1 is linked to hepatocellular carcinoma.