To investigate GLS1/GLS2 acetylation in murine HCC tumours, we collected mitochondrial lysates from DEN plus CCl4‐induced tumours and adjacent tissues and the acetylation levels of both GLS1 and GLS2 were significantly downregulated in tumours compared with adjacent tissues (Figure S4C,D). The gene discussed is GLS2; the disease is hepatocellular carcinoma.