To understand the clinical relevance of glutaminase activity, mTORC1 activation and GCN5L1 expression in human HCC, we examined glutaminase activities and the expression of p‐S6K, which indicates mTORC1 activation, and GCN5L1 in matched HCC specimens and adjacent normal liver tissues. This evidence concerns the gene RPS6KB1 and hepatocellular carcinoma.