In the primary fresh-frozen NSCLC tissue samples and the adjacent non-cancerous tissue, promoter DNA methylation was detected as follows: APC 82.8% and 85.7%, respectively, FOXA1 60% and 48.6%, respectively, SHOX2 65.7% and 42.8%, respectively, SLFN11 80% and 94.3%, respectively, and RASSFIA 60% and 45.7%, respectively. This evidence concerns the gene SHOX2 and non-small cell lung carcinoma.