Finally, in vivo experiments showed that ZCRB1, circHEATR5B, or HEATR5B-881aa overexpression could inhibit the growth of subcutaneous GBM xenograft tumors and prolong the survival time of nude mice with orthotopic GBM xenograft tumors; furthermore, their combination had the strongest tumor-suppressive effect. This evidence concerns the gene ZCRB1 and glioblastoma.