To further confirm the role of PI3K/Akt signaling pathway involved in regulating proliferation and gemcitabine resistance via hsa-miR-3178/RhoB axis in PC, PANC-1 cells and PANC-1-GEM cells were incubated with the PI3K inhibitor LY294002 and PI3K agonist 740Y-P. This evidence concerns the gene PIK3CB and pachyonychia congenita.