LENG9 and cervical carcinoma: For the overexpression assay, a full-length human LENG9 sequence was obtained by PCR and subcloned into the PLVX-puro vector by Lipofectamine 3000 to establish cells that stably overexpressed LENG9. Consistently, upregulation of LENG9 in CaSki and SiHa cells significantly increased proliferation, migration, and invasion of cervical cancer cells (Fig. 6d–f).