EZH2 and breast cancer: To explore the function of EZH2 in bone metastasis of breast cancer, we transfected either EZH2 shRNA or control shRNA into the MDA-MB-231 bone-seeking 231–1566 cell subline that expresses GFP and luciferase7 to generate the EZH2-knockdown cell lines 1566.shEZH2 and its control cell line 1566.shScr, respectively (Supplementary Fig. 1a).