Similar to the wild-type EZH2 re-expressing 231.KO#1.EZH2 cells, 231.KO#1.H689A cells that re-expressing EZH2 methyltransferase-dead mutant H689A had enhanced tumor cell proliferation and osteoclast maturation compared to 231.KO#1.pLenti cells (Fig. 2d, e). Here, EZH2 is linked to neoplasm.