Next, we examined whether EZH2 promoted bone metastasis outgrowth in vivo by increasing tumor cell proliferation or/and inhibiting apoptosis by IHC staining of Ki67 and cleaved caspase 3, which showed that the bone metastasis of EZH2 knockdown 1566.shEZH2 cells had significantly decreased proliferation and increased apoptosis compared to that of 1566.shScr cells (Supplementary Fig. 2h). This evidence concerns the gene CASP3 and neoplasm.