Similarly, downregulation of FAM64A suppressed the malignant activities of breast cancer cells, and the opposite findings were observed when FAM64A was overexpressed.22 Ectopic expression of FAM64A enhanced the proliferation, migration and invasion capabilities of osteosarcoma cells, and the tumor promoting effects of FAM64A were mediated by miR-493.23 FAM64A downregulation was also reported to suppress the proliferation of lung cancer cells.24 Although FAM64A is closely correlated with the malignant behaviors of cancer cells, the detailed molecular mechanisms are poorly understood. Here, PIMREG is linked to osteosarcoma.