In light of these few scattered reports about the proadipogenic effect of alkylglycerols, as well as the putative connection of AGMO and obesity including its comorbidities, we studied Agmo expression and activity in 3T3-L1 adipocyte differentiation and knocked down its expression by RNA interference in 3T3-L1 preadipocytes, monitored consequences on differentiation, and found quite unexpected impacts of decreased AGMO activity on the global cellular lipidome. This evidence concerns the gene AGMO and obesity disorder.