However, these factors are not related to the Wnt-independency phenotype in RNF43-mutant pancreatic cancer, since sgRNAs targeting KDM6A or negative regulators of GLI2 or YAP1, i.e., PTCH1, LATS1, and SAV1, were not enriched in ETC-159 versus vehicle-treated tumors (Figure 2F and Supplemental Figure 15B) in our initial CRISPR screens. This evidence concerns the gene GLI2 and familial pancreatic carcinoma.