In this study, through stringent and convincing lineage-tracing technology, we substantiated that neither AAV-shRNA- nor ASO-mediated astroglial PTBP1 repression could achieve AtoN or AtoDAn conversion either in the substantia nigra or in the striatum of a 6-OHDA mouse model of PD. This evidence concerns the gene PTBP1 and Parkinson disease.