Finally, we observed that Tcf4STOP/+::Neurod6-Cre mice used a similar amount of nest materials as their respective controls, while Tcf4STOP/+::Gad2-Cre mice used more nest materials than Tcf4STOP/+ mice, but significantly less material than controls (Figure 2E), demonstrating that embryonic reinstatement in glutamatergic neurons was sufficient to prevent the impaired nest building phenotype in PTHS model mice. The gene discussed is NEUROD6; the disease is Pitt-Hopkins syndrome.