To better assess the link between dysregulated SHP2 and myeloproliferation in the context of NS, we developed and characterized a novel genetic zebrafish model of NS with Shp2-D61G mutation, a dominantly inherited NS-associated mutation that is most frequently associated with NS/JMML-like MPN in human patients (Strullu et al., 2014; Tartaglia et al., 2001). Here, PTPN11 is linked to juvenile myelomonocytic leukemia.