Overall, the results improve our understanding of the widespread tryptophan variations and highlight the predominant role of tryptophan oxidation at GAPDH at 313 W, HBB at 16 W, and HBB at 38 W in regulating redox balance of cancer cells and their potential role as prognostic biomarkers in patients with NSCLC. This evidence concerns the gene GAPDH and non-small cell lung carcinoma.