These 35 OS-related NMRGs were then enrolled in the LASSO Cox regression analysis, finally constructing a NMRG prognosis signature for HCC patients based on the transcriptional profiling of selected 25 NMRGs (NDUFV2, NDUFAF1, COX15, LRPPRC, MPV17, CARS2, DARS2, GARS, HARS2, LARS, PARS2, VARS2, MTFMT, TRMT10C, TRMU, C12ORF65, MRPL3, FRDA, ISCU, COQ6, COQ7, PDSS1, CABC1, SPG7, and ATAD3), with the optimal value of λ (λ = 0.0106127) (Figure 3(b)). The gene discussed is DARS2; the disease is hepatocellular carcinoma.