The results showed that at 28 days tumor volumes of the DD3-ZD55-SPAG9, ZD55-SPAG9, DD3-ZD55-SPAG9, DTX, and PBS groups were 784.02 ± 145.59, 1003.21 ± 150.89, 1104.04 ± 110.39, 1352.00 ± 142.57, and 2321.36 ± 173.88. Here, SPAG9 is linked to neoplasm.