In this study, we found that LrB treatment suppressed the MI/R-induced upregulation of TGF-β1, TGF-β1R, and p-Smad2/3, revealing that LrB might attenuate MI/R-induced myocardial fibrosis via TGF-β1/Smad inactivation, which was correlated with the report of Bai et al. [13]. Here, SMAD2 is linked to Myocardial fibrosis.