Raising the cutoff up to 70 IU/L improved specificity (0.80, 95% CI: 0.72–0.87), but did not impaired sensitivity significantly (0.90, 95% CI: 0.73–0.98) owing to many pulmonary TB patients with neutrophil-dominant pleural effusion with ADA level over 70 IU/L who were additionally included in sensitivity analysis. This evidence concerns the gene ADA and pulmonary tuberculosis.