Limiting dilution assay (LDA) in vitro showed that the proportion of ZNF334-OE tumor stem cells was significantly lower than that of the control group, while the number of cell clusters formed by sh-ZNF334 was significantly higher than that of the control group, further indicating that overexpression of ZNF334 could reduce the tumor formation ability of tumor cells (Fig. 6A). This evidence concerns the gene ZNF334 and neoplasm.