Cox multivariate analysis found that AFP > 400 ng/ml, low ZNF334 expression and MVI were independent risk factors that affected the patients’ OS (Fig. 7E); while AFP > 400 ng/ml, low ZNF334 expression and portal vein tumor thrombus were the independent risk factors that affected patients’ RFS (Fig. 7F). This evidence concerns the gene ZNF334 and neoplasm.