ZNF334 and neoplasm: In addition, plate clone formation assay also showed that compared with ZNF334-NC, fewer tumor clusters with smaller size were formed in ZNF334-OE, while the clone formation ability of sh-ZNF334 was significantly higher than that of sh-NC, which further proved that overexpression of ZNF334 could inhibit the proliferation of tumor cells (Fig. 5M).