In conclusion, our study partly elucidated a sequential alternation of DNA methylation modification during the occurrence of HCC, provided some clues for revealing the temporal and spatial characteristics of DNA hypermethylation modification and firstly identified the regulatory mechanism of TP53 on its downstream target gene ZNF334. Furthermore, we explored the biological role of tumor suppressor gene ZNF334 in the occurrence of liver cancer and provided a new target and clinical evidence for the early diagnosis and precise treatment of liver cancer. This evidence concerns the gene ZNF334 and liver cancer.