Next, after 0, 6, and 24 h of infection with M. bovis in the two types of cells transfected with negative control and GBP2b-knockdown vectors, the AMPK, and ULK1 protein phosphorylation levels and mRNA levels were decreased after GBP2b knockdown, whereas the mTOR protein phosphorylation levels and mRNA levels were increased (Figure 6). The gene discussed is MTOR; the disease is infection.